INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Determination of pesticides and metabolites in wine samples by hplc-dad and second-order calibration methods
Autor/es:
BRAGA; BOTOLI; JARDIM; GOICOECHEA, HÉCTOR C; AC OLIVIERI,; POPPI
Revista:
JOURNAL OF CHROMATOGRAPHY - A
Editorial:
Elsevier
Referencias:
Año: 2007 vol. 1148 p. 200 - 210
ISSN:
0021-9673
Resumen:
The models parallel factor analysis (PARAFAC) and the recently introduced bilinear least squares (BLLS) were applied to develop second-order
calibration methods to high performance liquid chromatography with diode array detection (HPLC-DAD) data, where overlap of interferences
with the compounds of interest was observed, making the determination and resolution of the analytes possible. In this work, the simultaneous
determination of five pesticides and two metabolites in wine samples by HPLC-DAD was performed, using the second-order advantage. The results
of two chromatographic methods were compared, involving either isocratic or gradient elution. An appropriate preprocessing methodwas necessary
to correct the effects of time shifts, baseline variations and background. BLLS presented results that were of the same quality as PARAFAC in five
cases, but in two other situations only PARAFAC enabled analyte quantitation. Relative errors of prediction lower than 10% for all compounds
were obtained, indicating that the methodology employing HLPC-DAD and second-order calibration can handle complex analytical systems.