INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Simultaneous determination of rifampicin, isoniazid and pyrazinamide in tablet preparations by multivariate spectrophotometric calibration
Autor/es:
GOICOECHEA, HÉCTOR C; AC OLIVIERI,
Revista:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Editorial:
Elsevier
Referencias:
Año: 1999 vol. 20 p. 681 - 686
ISSN:
0731-7085
Resumen:
The use of multivariate spectrophotometric calibration is presented for the simultaneous determination of the active
components of tablets used in the treatment of pulmonary tuberculosis. The resolution of ternary mixtures of
rifampicin, isoniazid and pyrazinamide has been accomplished by using partial least squares (PLS-1) regression
analysis. Although the components show an important degree of spectral overlap, they have been simultaneously
determined with high accuracy and precision, rapidly and with no need of nonaqueous solvents for dissolving the
samples. No interference has been observed from the tablet excipients. A comparison is presented with the related
multivariate method of classical least squares (CLS) analysis, which is shown to yield less reliable results due to the
severe spectral overlap among the studied compounds. This is highlighted in the case of isoniazid, due to the small
absorbances measured for this component.