COUPLING ON LINE PRECONCENTRATIONS TO CAPILLARY ELECTROPHORESIS FOR TRACE METAL ANALYSIS

M. FERNANDA SILVA, SOLEDAD CERUTTI, RAÚL GIL, LUIS DANTE MARTÍNEZ, NORA M. VIZIOLI

Guaruja, Sao Pablo

Conferencia; XI Simposio Latinoamericano en Aplicaciones de la Electroforesis Capilar y Tecnología del Microchip en Biotecnología, Biomedicina , Biofarmacia e Industria; 2005

UNIVERSIDADE DO SAO PAULO

Modern analytical chemistry is characterized by hyphenated analytical methods which involve a combination of a separation and/or sensitive detection technique with and a  preconcentration step. The latter is a consequence of the problems encountered in the chemical analysis of real analytical samples such as low analyte concentrations (in many cases incompatible with quantification limits of modern instrumental methods) and high concentrations of matrix interference species present in these complex samples, especially those with biological or environmental origin . During the past two decades, capillary electrophoresis and related techniques have moved to the forefront of analytical chemistry and have excelled in the rapid analysis of ions replacing traditional separation methods.  Metal analysis by CE has the advantages of robustness and ruggedness, low-cost, rapidness and versatility. However, CE suffers from poor concentration sensitivity when using UV detection because of the small injection volumes (typically