INVESTIGADORES
LIJAVETZKY Diego Claudio
artículos
Título:
The Tata-less promoter of VP1, a plant gene controlling seed germination
Autor/es:
CARRARI, F; FRANKEL, N; LIJAVETZKY, D; BENECH-ARNOLD, R; SANCHEZ, R; IUSEM, N
Revista:
DNA Sequence
Referencias:
Año: 2001 vol. 12 p. 107 - 114
ISSN:
1042-5179
Resumen:
Vp1 is a seed-specific gene involved in the control of dormancy
and germination. We here present the complete sequence of the sorghum vp1
promoter/enhancer region highlighting its main features, especially the
lack of canonical TATA and CAAT boxes and the presence of elements
responsive to abscisic acid and light. The region closest to the start
of transcription is highly homologous to the partial proximal sequence
reported for the maize vp1 promoter. This region is interrupted
by a 57-nt stretch containing 14 CT microsatellite repeats. We observed
a poor overall homology to the promoter from abi3 gene, the Arabidopsis
counterpart bearing a similar coding sequence. However, there exists a
high degree of homology (89%) between a TATA-rich 103-bp stretch of the
sorghum vp1 promoter located about 700 nt upstream of the
startpoint and miniature inverted transposable elements (MITEs)
interspersed within the sorghum seed-specific kafirin cluster. This
sorghum MITE-like element displays considerable homology (68%) to the
TATA-less promoter from the sorghum NADP-malate dehydrogenase gene and
lesser similarity to the Tourist, Pilgrim and Batuta MITEs previously
identified within the promoter from the maize Abp1 (auxin-binding protein) gene.