INVESTIGADORES
LIJAVETZKY Diego Claudio
artículos
Título:
PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines
Autor/es:
HELGUERA, M; KHAN, IA; KOLMER, J; LIJAVETZKY, D; ZHONG-QI, L; DUBCOVSKY, J
Revista:
CROP SCIENCE
Referencias:
Año: 2003 vol. 43 p. 1839 - 1847
ISSN:
0011-183X
Resumen:
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Rust
resistance genes Lr37, Sr38, and Yr17 are located withina segment of Triticum ventricosum (Tausch) Cess. chromosome 2NS
translocated to the short arm of bread wheat chromosome 2AS.
Characterization of this chromosome segment by 13 restriction fragment
length polymorphism (RFLP) markers indicated that the 2NS
translocation replaced approximately half of the short arm of
chromosome 2A (distal 2538 centimorgans, cM). The objective of this
study was to develop polymerase chain reaction (PCR) assays based on
RFLP marker cMWG682 to facilitate the transfer of this cluster of
rust resistance genes into commercial wheat (Triticum aestivum
L.) cultivars. DNA sequence was obtained from the A-, B-, D-, and
N-alleles of cMWG682 and was used to design N-allele specific
primers. The 2NS fragment amplified by PCR primers cosegregated with
the presence of the RFLP-2NS band in all backcross populations. A
cleaved amplified polymorphic sequence (CAPS) was used to develop a
marker for the 2A-allele. This marker can be used to differentiate
homozygous and heterozygous plants carrying the 2NS translocation in
the final cycle of backcross introgression or in screenings for
homozygous plants in segregating populations. Finally, a third PCR
assay was developed by means of TaqMan technology as a
high-throughput alternative for selection of the 2NS/2AS
translocation in large segregating populations in breeding programs
that have access to real time PCR equipment. These molecular markers
were used to develop four hard red spring isogenic lines homozygous
for the 2NS chromosome segment. One of the isogenic lines, derived
from Anza, did not show the expected resistance in spite of the
presence of all the RFLP markers for the 2NS chromosome segment.
Analysis of F1 hybrids suggested that a suppressor of the
Lr37 gene is present in Anza. These isogenic lines will
provide a valuable tool to test the effects of the large 2NS
translocation on quality and agronomic performance.
