Distribution of photoactive β-carboline alkaloids across Passiflora caerulea floral organs

OSPINA-CALVO, BRIAN; DE GERÓNIMO, EDUARDO; VILLARRUEL, FERNANDO D.; APARICIO, VIRGINA C.; ASHWORTH, LORENA; ERRA-BALSELLS, ROSA; CABRERIZO, FRANCO M.

PHOTOCHEMISTRY AND PHOTOBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2023 vol. 00 p. 1 - 14

0031-8655

This study reports valuable information regarding the presence and concentration of a series of photoactive β-carboline (βCs) alkaloids (norharmane, harmane, harmine, harmol, harmaline, and harmalol) and their distribution across the floral age and organs of Passiflora caerulea. UHPLC–MS/MS data reported herein reveal that the βCs´ content ranged from 1 to 110 μg kg−1, depending on the floral organ and age. In certain physiologically relevant organs, such as anthers, βCs´ content was one order of magnitude higher than in other organs, suggesting a special role for βCs in this specific organ. βCs´ content also varied in a structure-dependent manner. Alkaloids bearing a hydroxyl group at position C(7) of the main βC ring were present at concentrations one order of magnitude higher than other βC derivatives investigated. UV–visible and fluorescence spectroscopy of the flower extracts provided complementary information regarding other biologically relevant groups of chromophores (phenolic/indolic derivatives, flavonoids/carotenes, and chlorophylls). Since flowers are constantly exposed to solar radiation, the presence of photoactive βCs in floral organs may have several (photo)biological implications that are further discussed.