INVESTIGADORES
BUZZOLA Fernanda Roxana
congresos y reuniones científicas
Título:
Comparing initial and within-host adapted Staphylococcus aureus during the course of a persistent bovine mastitis.
Autor/es:
MARBACH H; MAYER K; BUZZOLA, FR; SORDELLI DO; EHLING-SCHULZ, M; GRUNERT, T
Lugar:
Amsterdan, Holanda
Reunión:
Congreso; 29th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID).; 2019
Resumen:
Our work demonstrates that S. aureus within-host adaptation to the bovine mammary gland favores the transition towards a SigB-deficient pathotype associated with a single nucleotide polymorphism in rsbU (G368A → G122D), a contributor to SigB-functionality. Along with phenotypic features, typical for sigB-deficiency, the emergence of the host-adapted isolate implies a selective advantage associated with distinct pathotypic traits, allowing it to presumably better survive extracellular within the mammary gland: strong increased protease activity and production of poly-N-acetylglucosamine (PNAG)-based biofilm. Importantly, the SigB-deficient pathotype also showed a significantly decreased virulence in a mouse model of infection, indicating a switch towards reduced virulence during infection. In conclusion, our work fosters the hypothesis that the sigB-regulon is most crucial for S. aureus adaptation during chronic infection.