Phenotypic and genotypic characterization of Streptococcus uberisisolated from bovine subclinical mastitis in Argentinean dairy farms

LASAGNO MC; REINOSO EB; DIESER SA; CALVINHO, LF; BUZZOLA FR; VISSIO C; BOGNI CI; ODIERNO L

REVISTA ARGENTINA DE MICROBIOLOGIA.

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2011 vol. 43 p. 212 - 217

The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberiswere isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. TheS. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profileswere identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similaritywere identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberisstrains with the same PFGE pattern showed different virulence profiles. Bovine S. uberisstrains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypiccharacteristics, and the PFGE patternsare not associated with the virulence profiles.