Polyelectrolytes protein complexes: a viable platform in the downstream processes of industrial enzymes at scaling up level

M EMILIA BRASSESCO; NADIA WOITOVICH VALETTI; GUILLERMO PICÓ

J Chem Technol Biotechnol

Wiley Online Library:

Lugar: London; Año: 2016

1097-4660

Complexation between proteins and polyelectrolytes through electrostatic interactions gives either soluble or non-solublecomplexes, leading to phase separation. The formation and the stability of these complexes are influenced by pH, ionicstrength, ratio between protein and polyelectrolyte and charge density of protein and polyelectrolyte. This review presentedthe advantages on the protein precipitation by adding a polyelectrolyte solution to a natural enzyme source (microbiological,vegetal or animal homogenate), over the classical precipitation methods. An enzyme can be precipitated like non-solublecomplex, separated and then re-dissolved, thus the method works, at the same time, like concentrative and pre-purificationsteps together.