Adsorption of peroxidase from Raphanus sativus L onto alginate guar gum matrix: kinetic, equilibrium and thermodynamic analysis.

ANA SILVA; NADIA WOITOVICH VALETTI; MARIA EMILIA BRASSESCO; JOSE TEIXEIRA; GUILLERMO PICÓ

ADSORPTION SCIENCE & TECHNOLOGY

MULTI SCIENCE PUBL CO LTD

Lugar: Brentwood, UK; Año: 2016 vol. 23 p. 388 - 402

0263-6174

This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption ontospherical guar gum?alginate matrices. The effect of contact time, solution pH, initial proteinconcentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, andwas higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order ratekinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzedwith different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical andunspecific interaction between the enzyme and the matrix.