Obtainment of a highly concentrated pancreatic serine proteases extract from bovine pancreas by precipitation with polyacrylate

J. LOMBARDI; NADIA WOITOVICH VALETTI; G, PICÓ; BOERIS, V

SEPARATION AND PURIFICATION TECHNOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 16 p. 170 - 174

1383-5866

Serine proteases have wide application in leather, food, meat and soap powder industries, among others. 28 There are a lot of methodologies to obtain them in large quantities but most of them are expensive or 29 contaminating.We then propose an economical and environmentally friendly method in which proteases 30 are separated from a crude fresh bovine pancreas homogenate using precipitation with polyacrylate, a 31 commercially available negatively charged and weak polyelectrolyte. The zymogens of the serine prote- 32 ases were activated prior to precipitation by the addition of trypsin. The proteases were precipitated by 33 adding polyacrylate at pH 4.50 to the pancreas homogenate. Under these conditions, serine proteases are 34 positively charged and form non-soluble complexes with the negatively charged polyacrylate. The purity 35 of proteases was increased 5-fold with a recovery of 33% under the best conditions tested. The volume of 36 the final product was decreased to 5% of the feedstock, in order to concentrate the sample up to 20 times. 37 The proposed method removed up to 96% of the contaminant proteins. 38