Isolation and characterization of microsatellite loci for the southern geoduck, Panopea abbreviata (Valenciennes, 1839) through 454 pyrosequencing

LÓPEZ-MÁRQUEZ, V; FERNÁNDEZ ALFAYA, J.E.; GARCÍA- JIMÉNEZ, R; BIGATTI G.; MACHORDOM, A.

MOLECULAR ECOLOGY RESOURCES

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2013 vol. 13 p. 1 - 12

1755-098X

We have isolated the first, polymorphic microsatellite loci (21 in total) for the geoduckclam Panopea abbreviata (Valenciennes, 1839) from San Matías gulf (Patagonia, Argentina), using 454 GS-FLX Titanium pyrosequencing. We also developed conditions for amplifying these markers in 5 multiplex and 7 individual reactions. Four to 23 alleles were detected per locus across the 25 samples analysed. Observed and expected heterozygosities ranged from 0.235 to 0.985 and from 0.528 to 0.937, respectively. In the sampled population, only one locus deviated from Hardy?Weinberg equilibrium. These markers are useful resources for future population structure studies in this artisanal fishing species