Model lipidic membrane as a Losartan bioindicator

FLORENCIA SAN ROMÁN NÁPOLI; CAROLINA ACEBAL; MARÍA BELÉN SIERRA

Buenos Aires

Congreso; XLIX Reunión Anual SAB; 2021

SOCIEDAD ARGENTINA DE BIOFISICA

Liposomes formed by phospholipids can be used as model membranes to study thebehavior of biological membranes because they show similarities such as their structure,composition, and selective permeability [1]. Therefore, lipidic membranes can also beused to predict the effects of exposure of living organisms to contaminants of emergingconcern (CECs) [2-4]. CECs are bioaccumulative compounds of different chemical naturethat can be potentially toxic to the environment, and they have gone unnoticed due tolack of information or adequate techniques for their identification. Among them areproducts such as: drugs, pesticides, cosmetics, cleaning supplies and personal hygieneamong others.The liposome becomes a bioindicator that will provide information on the toxicity andinteraction of CECs with cell membranes, detecting the action of emerging pollutants onliving organisms. In this way, the impact of toxic substances in terms of bioaccumulationand bioavailability in the body can be anticipated, using a membrane model that mimicsthe cell membrane, minimizing the use of biological membranes and living beings forexperimentation.In this work, the interaction of an emerging contaminant present in water was explored:Losartan, an antihypertensive agent, with a mixed model membrane composed ofphosphatidylcholines: DPPC-POPC (0.75: 0.25) through studies of zeta potential, particlesize, conductivity, density, and speed of sound. It was observed that the presence ofLosartan in the water affects the surface and mechanical properties of the modelmembrane.The results are expected to provide insight into the toxicity, bioavailability, andbioaccumulation of emerging pollutants in living beings.References[1] C Peetla et al, Mol Pharm. 6, 5 (2009) 1264[2] H Wang et al, ACS Omega 3, 11 (2018) 15353[3] M Oliver et al, Science of the Total Environment 738 (2020) 140096[4] M Oliver et al, Talanta 209 (2020) 120600