Effect of mobile ions in the electron transfer process in enzyme based amperometric sensors.

DANIELE ZAPPI; FERNANDO BATTAGLINI

Bologna

Congreso; XXVII Congresso della Divisione di Chimica Analitica della Società Chimica Italiana.; 2018

Societa Chimica Italiana

Polyallylamine (PA) is a weak polyelectrolyte widely used in materials science because of its ductility as working probe. Its interaction with polyelectrolytes of opposite charge allows to create multilayer polyelectrolytes forming highly stable interfaces. Ionic strength of the solutions is thus critical in determining the properties of the resulting structure. Small cations and anions (called mobiles), can form ionic pairs with charged sites on the polyelectrolyte chains to reduce their intra-chain electrostatic repulsions promoting the formation of ball-like conformations. In this work is presented the use of PA, modified with an osmium polypyridine complex (OsPA), assembled in presence of chloride, sulfate or phosphate ions, using as counter charged layer Glucose Oxidase enzyme (GOx).