Digital simulation of homogenous enzyme kinetics for amperometric redox enzyme electrodes

FERNANDO BATTAGLINI; ERNESTO J. CALVO

ANALYTICA CHIMICA ACTA

Elsevier

Lugar: Amsterdam; Año: 1992 vol. 258 p. 151 - 160

0003-2670

An explicit point method is described for digital simulation of redox-enzyme catalysis and diffusion at soluble-enzyme electrodes. Two experimental conditions (chronopotentiometry and open-circuit potential decay) were simulated for a two-substrate enzyme (glucose oxidase) catalyzing the anaerobic oxidation of glucose, mediated by a soluble redox couple. Transient and steady-state responses are compared to experimental data and to predictions of the analytical solutions for selected cases. Simulated steady-state concentration profiles near the electrode surface are analyzed for different values of Ks/Cs and KO/CO. The validity of linear Lineweaver—Burk plots for the amperometric response of enzyme electrodes is discussed.