THIRD ORDER MULTIVARIATE CALIBRATION APPLIED TO THE QUANTITATION OF AZINPHOS-METHYL IN FRUITS BY EXCITATIONEMISSION- KINETIC MEASUREMENTS

MONTEMURRO M; SIANO G G; CULZONI; GOICOECHEA H C

Congreso; XVIII CAC 2016; 2016

Azinphos-methyl (AZM) is a widely used organophosphate insecticide and acaricide, with demonstratednegative impacts on the environment [1]. The weak natural fluorescence of AZM in aqueous solutioncan be enhanced in a variety of ways, including UV photolysis, inclusion into cyclodextrins, and basehydrolysis. Upon absorption of UV-A radiation, this molecule undergoes photolysis to the highlyfluorescent compound N-methylanthranilic acid, which undergoes subsequent photolysis tophotochemically stable products [2].Four-way data generated by excitation-emission fluorescence matrices (EEFMs) measured as a functionof reaction time can be modelled with appropriate higher order algorithms to achieve the second orderadvantage and improve both the selectivity and sensibility of the method.In this work, we developed a fluorescent kinetic method for the determination of AZM in fruit samples.It is based on third-order data, obtained by measuring the time evolution of the EEFMs of the photolysisof AZM in alkaline medium.