Science based calibration for the extraction of ‘analyte-specific’ HPLC-DAD chromatograms in environmental analysis

J. KULIGOWSKI; MARTINEZ GALERA, MARIA; GIL GARCÍA, MARÍA; CULZONI M J; GOICOECHEA, H C; G. QUINTÁS; M. DE LA GUARDIA

TALANTA

ELSEVIER SCIENCE BV

Año: 2011 vol. 83 p. 1158 - 1165

0039-9140

Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks inliquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaksfrom interfering matrix compounds and a change in the background absorbance due to the mobile phasegradient. Applying the present multivariate approach it was possible to resolve all 11 analytes fromoverlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samplesranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentrationranges considered to 1–7gL−1.