Determination of six veterinary pharmaceuticals in egg by liquid chromatography: chemometric optimization of a novel air assisted-dispersive liquid-liquid microextraction by solid floating organic drop

TEGLIA, CARLA M.; GONZALO, LUCIA; CULZONI, MARÍA J.; GOICOECHEA, HÉCTOR C.

FOOD CHEMISTRY

ELSEVIER SCI LTD

Año: 2019 vol. 273 p. 194 - 202

0308-8146

Two extraction strategies for albendazole, chloramphenicol, trimethoprim, enrofloxacin, oxitetracycline and nicarbazin in egg were optimized for its quantitation by liquid chromatography. First, to find out the optimized condition for the air assisted-dispersive liquid-liquid microextraction based on solidification of organic drop, two designs were built: a fractional factorial and a central composite design. The optimum conditions resulted 1140 µL of water, 125 mg of ZnSO4, 1175 µL of acetonitrile, 1200 µL of methanol and 740 µL of acetone, using 1.00 g of homogenized egg and 50 µL of 1-dodecanol. Second, two designs were also built to optimize the dispersive liquid-liquid microextraction: a central composite and a mixture designs to set the combination of the re-suspended solvents. The optimum conditions resulted 1840 μL of acetonitrile and 160 μL of dichloromethane and the re-suspended mixture of acetonitrile and sodium phosphate buffer 10 mmol L?1 pH = 3.50 (30:70 v/v).