Solving matrix-effects exploiting the second order advantage in the resolution and determination of eight tetracycline antibiotics in effluent wastewater by modelling liquid chromatography data with multivariate curve resolution-alternating least squares

GIL GARCÍA, MARÍA; CULZONI MARÍA JULIA; DE ZAN,; SANTIAGO VALVERDE, ROSARIO; MARTINEZ GALERA, MARIA; GOICOECHEA, HÉCTOR C

JOURNAL OF CHROMATOGRAPHY - A

Elsevier

Año: 2008 vol. 1179 p. 115 - 124

0021-9673

A new powerful algorithm [unfolded-partial least squares followed by residual bilinearization (U-PLS/RBL)] was applied for first time on second-order HPLC-DAD data and compared with a well known established method [multivariate curve resolution-alternating least squares (MCR-ALS)] for the simultaneous determination of eight tetracyclines (tetracycline, oxytetracycline, meclocycline, minocycline, metacycline, chlortetracycline, demeclocycline and doxycycline) in wastewaters. Tetracyclines were pre-concentrated using OASIS MAX C18 cartridges and then separated on a Thermo AQUASIL C18 (150 ´ 4.6 mm, 5 μm). The whole method was validated using Milli-Q water samples and both univariate and multivariate analytical figures of merit were obtained. Additionally, two data pre-treatment were applied (baseline correction and piecewise direct standardization), which allowed to correct the effect of breaktrough and to reduce the total interferences retained after preconcentration of wastewaters. The results showed that the eight tetracycline antibiotics can be successfully determined in wastewaters, the drawbacks due to matrix interferences being adequately handled and overcome by using U-PSL/RBL.