Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences

VERA CANDIOTI, LUCIANA; CULZONI MARÍA JULIA; AC OLIVIERI,; GOICOECHEA, HÉCTOR C

ELECTROPHORESIS

Whiley

Lugar: Londres; Año: 2008 vol. 29 p. 4527 - 4537

0173-0835

Drug monitoring in serum samples was performed using second-order data generated byCE-DAD, processed with a suitable chemometric strategy. Carbamazepine could beaccurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additionalserum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and(iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, itsmetabolite, lamotrigine and unexpected serum components) by the well-knownmultivariate curve resolution-alternating least squares algorithm, which extractsquantitative information that can be uniquely ascribed to the analyte of interest. Theanalyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoverieswere 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracyand precision of the proposed method