Room temperature fluorescence spectroscopy of Benzo[a]pyrene metabolites on octadecyl extraction membranes

BASSAM ALFARHANI; MAHA AL-TAMEEMI; SCHENONE A V; BARBOSA F; GOICOECHEA HC; ANDRES CAMPIGLIA,

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 129 p. 83 - 89

0026-265X

Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon(PAH), which requires metabolic activation to produce its detrimental effects. Measurement ofB[a]P metabolites in human urine could provide a direct way to assess individual differences insusceptibility to PAH-related cancer. This article focuses on the development of screeningmethodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solidsurfaceroom-temperature fluorescence (RTF) properties of 3-hydroxy-benzo[a]pyrene,benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol andbenzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples withoctadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6 % (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiberoptic probe that eliminates the need for manual optimization of signal intensities. Analyticalrecoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to99.8 ± 2.5 % (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figuresof merit and the simplicity of the experimental procedure demonstrate the potential of thisapproach for screening biomarkers of PAH exposure in numerous urine samples