Ultra fast quantitation of six quinolones in water samples by second order capillary electrophoresis data modeling with multivariate curve resolution?alternating least squares

ALACARÁZ, M; VERA CANDIOTI, L; CULZONI M J; GOICOECHEA H C

ANALYTICAL AND BIOANALYTICAL CHEMISTRY

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2014

1618-2642

This paper presents the development of a capillary electrophoresis method with DAD coupled to multivariate curve resolution-alternating least-squares (MCR?ALS) to carry out the resolution and quantitation of a mixture of six quinolones in the presence of several unexpected components. Overlapping of time profiles between analytes and water matrix interferences were mathematically solved by data modeling with the well-known MCR?ALS algorithm. With the aim of overcoming the drawback originated by two compounds with similar spectra, an especial strategy was implemented to model the complete electropherogram instead of dividing the data in region as usually performed in previous works. The method was firstly applied to quantitate analytes in standard mixtures which were randomly prepared in ultrapure water. Then, real water samples spiked with several interferences were analyzed. Recoveries between 76.7 and 125%, and LODs between 5 to 18 g L?1 were achieved.