ARAMENDIA Pedro Francisco
congresos y reuniones científicas
New aza-boron-dipyrromethene compounds for single molecule fluorescence studies
ALAN SZALAI; LUCIANA GIORDANO; SARA BARI; SUSANA SILBERSTEIN; CLAUDIO N. CAVASOTTO; PEDRO F. ARAMENDÍA
Congreso; 25th Inter-American Photochemical Society Meeting; 2016
Inter-American Photochemical Society
Corticotropin-releasing hormone (CRH) is a key peptide involved in neuroendocrine responseto stress. There is a widespread interest in understanding CRH type 1 receptor (CRHR1)molecular behavior, as it plays a primary role in regulatory mechanisms1. Owing to the lackof reliable specific antibodies for CRHR1, studying its dynamics and cellular distribution byfluorescence microscopy requires a different labeling strategy. A set of novel fluorescentantagonists have been designed for this purpose. Based on non-fluorescent reportedantagonists and the crystal structure of CRHR12, a set of compounds based on an aza-borondipyrromethene(aza-BODIPY) core were chosen for a docking study. Aza-BODIPYs aresuitable for single molecule fluorescence microscopy (SMFM), due to their highphotostability and emission brightness3. Docking studies were performed using the InternalCoordinates Mechanics (ICM) software (MolSoft LLC, La Jolla, CA), and a flexible ligandrigidreceptor approach. Compound 1 was chosen as the first compound to be synthesized. Itsprecursor, 2, has been successfully synthesized and its spectroscopic properties weremeasured.