Chromosome Data in Paspalum lilloi

REUTEMANN, AV; MARTINEZ, EJ; SCHEDLER, M; RUA, GH; DAVIÑA, JR; HONFI, AI

TAXON

INT ASSOC PLANT TAXONOMY

Lugar: Viena; Año: 2017 vol. 66 p. 1487 - 1499

0040-0262

Paspalum lilloi Hack. is an endemic species from Iguazú Falls.Karyomorphometric analyses and meiotic behaviour description arepresented for the first time for the species. It showed unimodal andsymmetrical karyotype (1A according to Stebbins?s categories, 1971),comprising 20 metacentric chromosomes (Fig. 8A) whose length isranging from 2.8 to 1.6 μm, and 22.69 μm per haploid genome. Chromosomesbehave regularly at meiosis, with mainly 20II at diakinesisand also 9II + 2I, 8II + 4I, 7II + 6I, 6II + 8I (Fig. 8B). At diakinesis andmetaphase I, the mean frequency of I was 3.085 ± 0.358 per pollenmother cell (PMC) and 8.457 ± 0.209 II per PMC.Tri-staining with CMA/Distamycin-A/DAPI fluorochromesrevealed the presence of two pairs of bands localized on two microsatellites,in pair 3 and 9, respectively. Chromosomes were arranged, accordingto the total chromosome length. The microsatellite of the shortarm on pair 3 was GC- rich (CMA+/DAPI0), and a GC-rich (CMA+)terminal band on the long arm of these chromosomes was detected. Thepair 9 showed an AT-rich band as well as a GC-rich (CMA+/DAPI+)microsatellites, both localized on the short arm (Fig. 8C, D).Karyotype, and heterochromatin pattern are presented for thefirst time for the species.