INVESTIGADORES
ANGEL Sergio Oscar
artículos
Título:
Use of Leishmania infantum heat shock protein (Hsp) 83 in the serodiagnosis of tegumentar leishmaniasis
Autor/es:
CELESTE, B.; SERGIO OSCAR ANGEL; CASTRO, L.; GIDLUND, M.; GOTO, H.
Revista:
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
Editorial:
ASSOC BRAS DIVULG CIENTIFICA
Referencias:
Año: 2004 vol. 37 p. 1591 - 1593
ISSN:
0100-879X
Resumen:
The serologic assay is an important tool in the diagnosis of leishmaniasis.
One of the most commonly used tests is enzyme-linked immunosorbent
assay (ELISA). Since total Leishmania promastigotes are used
as antigen in the routine assay, false-positive reactions are frequent
due to cross-reaction with sera from other diseases, mainly Chagas
disease. Therefore, an antigen that determines less cross-reactivity has
been pursued for the serodiagnosis of leishmaniasis. In the present
study we analyzed the use of recombinant Leishmania infantum heat
shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous
(N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed
the presence of anti-L. infantum Hsp 83 antibodies in all samples as
well as anti-Leishmania total antigen antibodies. When cross-reactivity
was tested, chronic Chagas disease patients (N = 10) did not show
any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a
good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.Leishmania promastigotes are used
as antigen in the routine assay, false-positive reactions are frequent
due to cross-reaction with sera from other diseases, mainly Chagas
disease. Therefore, an antigen that determines less cross-reactivity has
been pursued for the serodiagnosis of leishmaniasis. In the present
study we analyzed the use of recombinant Leishmania infantum heat
shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous
(N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed
the presence of anti-L. infantum Hsp 83 antibodies in all samples as
well as anti-Leishmania total antigen antibodies. When cross-reactivity
was tested, chronic Chagas disease patients (N = 10) did not show
any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a
good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.Leishmania infantum heat
shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous
(N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed
the presence of anti-L. infantum Hsp 83 antibodies in all samples as
well as anti-Leishmania total antigen antibodies. When cross-reactivity
was tested, chronic Chagas disease patients (N = 10) did not show
any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a
good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.L. infantum Hsp 83 antibodies in all samples as
well as anti-Leishmania total antigen antibodies. When cross-reactivity
was tested, chronic Chagas disease patients (N = 10) did not show
any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a
good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.Leishmania total antigen antibodies. When cross-reactivity
was tested, chronic Chagas disease patients (N = 10) did not show
any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a
good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.L. infantum Hsp 83 to be a
good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.