INVESTIGADORES
ANGEL sergio Oscar
artículos
Título:
Otimizaçao de polimerase em cadeia para detecçao de Toxoplasma gondii em sangue venoso e placenta de gestantes.
Autor/es:
SPALDING, S.; AMENDOEIRA, MRR; COELHO, J.; SERGIO OSCAR ANGEL
Revista:
JORNAL BRASILEIRO DE PATOLOGIA E MEDICINA LABORATORIAL
Referencias:
Año: 2002 vol. 38 p. 105 - 110
ISSN:
1676-2444
Resumen:
The Toxoplasma gondii detection in venous blood and placenta of pregnants by polymerase
chain reaction may facilitate the diagnosis of congenital toxoplasmosis. We evaluated pregnants
with reagent immunoglobulin M (IgM) and their children. Beyond serological toxoplasma-specific
immunoglobulins G (IgG), M (IgM), A (IgA) and IgG avidity (MEIA), we did immunoperoxidasis
and mice inoculation. Genomic amplification with gene B1 primers and new TgR primers (called
ABGTg7 C1 and N1) was conducted for each sample. Its necessary to observe that treatment
probably is responsible for the infection decrease, that manner, negative results would confirm
uteri therapeutic efficiency. PCR was sensitive and specific, it identified one to ten tachyzoites.
Besides being a fast form of congenital toxoplasmosis diagnosis, it may be used safely and trustly,
as an important tool for congenital evaluation.