A new antitubercular mulinane diterpenoid from Azorella madreporica Clos

WÄCHTER, GERALD A.; FRANZBLAU, SCOTT G.; MONTENEGRO, GLORIA; SUAREZ, ENRIQUE; FORTUNATO, RENÉE H.; SAAVEDRA, EDGARDO; TIMMERMANN, BARBARA N.

JOURNAL OF NATURAL PRODUCTS

AMER CHEMICAL SOC

Lugar: WASHINGTON; Año: 1998 vol. 61 p. 965 - 968

0163-3864

Bioactivity-guided fractionation of the petroleum ether extract of Azorella madreporica Clos has led to the isolation of the novel, antitubercular mulinane diterpenoid 1. The structure has been elucidated on the basis of its 1D and 2D NMR spectra and by comparison with mulinolic acid 2 and a dehydration product 3 obtained from 1. The MIC of 1 for growth inhibition of the H37Rv strain of Mycobacterium tuberculosis was determined as 20 μg/mL. LC-MS and NMR have suggested the presence of this new compound in four other species of Azorella.