Exploring magnetic hyperthermia: ferrofluids, in vitro and in vivo experiments

DIEGO CORAL; PAULA SOTO; VIVIANA BLANK; PEDRO MENDOZA ZELIS; GUSTAVO ALBERTO PASQUEVICH; SERGIO GONZALEZ; ENRIQUE MARIO SPINELLI; ALEJANDRO LUIS VEIGA; FRANCISCO HOMERO SÁNCHEZ; PATRICIA CLARA SETTON; LEONOR P. ROGUIN; MARCELA BEATRIZ FERNÁNDEZ VAN RAAP

Gramado

Congreso; XVI Brazil MRS Meeting; 2017

Brazilian Materials Research Society

Therapeutic and diagnostic methodologies based in nanotechnology are ofimportance nowadays. These protocols are meant be less invasive, more efficient and displaying minor side effects, being endowed of Selectivity. Magnetic hyperthermia (MH), a modality that uses radio-frequency (RF) magnetic fields to heat single-domain magnetic nanoparticles (NPs), is becoming a powerful oncological therapy. Iron oxide NPs are the most biocompatible materials accepted as a medical device. MH has reached clinical trial, but there are still unsolved problems like: dosage; NPs spatial distribution in target tissue andtemperature distribution, monitoring and increase control. Dosage is mainly determined by NPs´s efficiency to transduce heat (SAR).This efficiency depends on magnetic relaxation and it is highly influenced by NP size, aggregation and interaction. It is further modified by estricted mobility and confinement inside tumour environment. We have performed MH experiments in ferrofluids [1,2], in vitro [3] and more recently in vivo mice assays. We have analyzed aqueous dispersion of single NPs and randomly or magnetically organized multicore. We proved that the relevant physical parameters for predicting fluid SAR are mean NP volume, its saturation magnetization and a representative mean activation energy, and that SAR depends on concentration [2,4]. In vitro experiments were used to determine uptake, distribution, cytotoxicity and to develop a new methodology for probing intracellular heating in cells cultures [3]. In vivo constitute a more complex scenario because after intratumoral infiltration irregular NP distribution patterns occurs.Also we will present a new portable device, optimized to generate fields of 100 kHz and amplitude from 2 to 15 kA/m, versatile for MH research in materials, cell cultures and mice models.[5].