INVESTIGADORES
MIGUEL virginia
congresos y reuniones científicas
Título:
Interaction of the C-terminal region of P. aeruginosa MutS with the Dnak chaperone
Autor/es:
MONTI MR; MIGUEL V; ARGARAÑA CE
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; XLV Reunión Anual de SAIB; 2009
Institución organizadora:
SAIB
Resumen:
MutS recognizes misspaired bases and it is
required to activate downstream events in the postreplicative DNA Mismatch
Repair System. In a previous work, we determined that the C-terminal region of P.
aeruginosa MutS is involved in the interaction with other factor necessary
for the correct function of the MRS. To analyze if the C-terminal region of
MutS (Cter) interacts with other protein, the DNA encoding this domain was
fused in frame to the maltose binding protein (MBP) gene, and expressed in P.aeruginosa.
The purified MBP-Cter protein consistently copurified with the chaperone DnaK,
which was absent in MBP purified preparations. The P. aeruginosa cells
expressing the fusion MBPCter acquired a phenotype similar to that previously
described for dnaK mutants in other bacteria: diminished viability, cell
filamentation and high temperature sensitivity. Similar phenotypes were
detected by overexpressing the full length MutS protein. On the contrary,
overexpression of a C-terminal deletion MutS mutant did not produce phenotypic
changes compared to the wild type strain. We propose that DnaK interacts with
the C-terminal region of MutS and the overexpression of the repair protein
produces a complete sequestration of DnaK. We are currently analyzing the interaction
MutS-DnaK in vitro and generating P. aeruginosa dnaK mutant strains to
characterize its phenotype.