INVESTIGADORES
FONSECA Maria Isabel
congresos y reuniones científicas
Título:
Design of degenerated primers from bioinformatics online software for putative ACE-1 trancription factor in Peniophora sp
Autor/es:
ANA BELÉN RAMOS HRYB; MARIA ISABEL FONSECA; LAURA VILLALBA; PEDRO DARIO ZAPATA
Lugar:
Quilmes
Reunión:
Congreso; 1er Congreso Argentino de Bioinformática y Biología Computacional. online: http://www.slideshare.net/a2b2c/ramos-ace1; 2010
Institución organizadora:
Universidad Nacional de Quilmes
Resumen:
Cellulose-paper industries have high environmental impact, that is why there are efforts to reduce pollution. In this sense white rot fungi such as Peniophora sp (BAFC 633) present enzymes with wide degradative capacity, like Laccase (Lac) [1], with potential to alleviate environmental problems. Several cupper-binding sites in Lac have been discovered [2] and the presence of DNA sites coding transcription factor in response to Cu+2 (ACE-1) in Phanerochaete chrysosporium (Genbank accession number BF60559.1) has been demonstrated [3], which could activate the laccase gene transcription [4]. The objective of this study was to define conserved regions using bioinformatics tools and to design degenerated primers to amplify a segment of a putative region coding ACE-1 in Peniophora sp. genome.