Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic Escherichia coli challenge

THELMA VERONICA POGGIO; LA TORRE JOSE; EDUARDO ALBERTO SCODELLER

CANADIAN JOURNAL OF MICROBIOLOGY

NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Lugar: Otawa; Año: 2006 vol. 52 p. 1093 - 1102

0008-4166

In this work we compared the efficacy of an experimental intranasal vaccine against urinary tract infections (UTI) containing a recombinant truncated FimH adhesin (rFimHt) plus CpG oligodeoxynucleotides with an intramuscular vaccine formulated with the same immunogen plus Freund?s adjuvant. Our results show that serum IgG titers of vaccinated animals were similarly enhanced in both cases. However the intranasal vaccine elicited higher vaginal wash specific IgA titer against rFimHt compared to the intramuscular route. Both vaccines reduced in vivo colonization of bladder by uropathogenic Escherichia coli (UPEC) by more than 2 Log in a murine cystitis model. Our results suggest that immunization with rFimHt adhesin plus CpG is a suitable immunogenic combination that can contribute to the development of a highly efficacious urinary tract infection vaccine.