A Maturase K-like transcript is regulated by auxins in Arabidopsis thaliana seedlings

DISTÉFANO A.; DURAND D.; MUCCI V.; PAGNUSSAT L.; RAMELLA N.; TUROWSKI V.; PARIS R.; GODOY V.; LAMATTINA L.

Iguazú, Misiones.

Congreso; XL Reunión Anual Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB).; 2004

Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

These authors contributed equally to the present work. Auxins are hormones that mediate a variety of developmental processes in plants. The precise molecular mechanisms involved in the hormone actions remains uncertain. Recently, it has been described that nitric oxide (NO) in plants is involved in some well-defined auxin-promoted physiological responses. The objetive of this work was to identify changes in the transcriptoma profile of A. thaliana treated with NO and to study if they were involved in auxin signaling pathway. Thereby we developed a differential display (DD) assay on seedlings treated for 12 and 24h with the NO donor. Seven differentially expressed cDNAs were detected from the DD analysis in NO-treated seedlings. We selected one of them (At04) to use it as a probe for a Northern blot analysis of NO-and 2.4-D-trated seedlings. Surprisingly At04 was up-regulated in 2.4-D-treated seedlings, but remained unmodified by NO. The nucleotide sequence revealed that At04 shares high homology with a chloroplastic maturase K cDNA. The maturases are proteins involved in the regulation of splicing of certain mRNAs. Further analysis will be presented in order to integrate know auxin- and NO-signaling pathways with the role of maturases in the control of plant gene expression.