Secretomic analysis of cheap enzymatic cocktails of Aspergillus niger LBM 134 grown on cassava bagasse and sugarcane bagasse

DIAZ GABRIELA V; CONIGLIO ROMINA O; ALVARENGA, AE;; ZAPATA, PD.,; VILLALBA L. L.,; FONSECA MI

MYCOLOGIA.

ALLEN PRESS INC

Lugar: Lawrence; Año: 2020

0027-5514

Currently, agroindustrial wastes are little used for generating value-added products; hence, theiruse of these waste to produce enzymatic cocktails for the conversion of lignocellulosic biomass to10 fermentable sugars is a very interesting alternative in the second-generation bioethanol process.The Ascomycota fungus Aspergillus niger LBM 134 produces hydrolytic enzymes in large proportions.In this work, A. niger LBM 134 was grown on sugarcane and cassava bagasses under optimizedconditions. To identify the extracellular enzymes involved in the degradation of these agroindustrialwastes, the secretomes of the culture supernatants of the fungus were analyzed and validated by15 biochemical assays of the enzymatic activities. A. niger LBM 134 secreted higher quantities ofxylanases and accessory hemicellulases when it grew on sugarcane bagasse, whereas more cellulases,amylases, and pectinases were secreted when it grew on cassava bagasse. These findingssuggest two promising enzyme cocktails for the hydrolysis of lignocellulose carbohydrate polymersto fermentable sugars. These bioinformatic analysis were functional validates through enzymatic20 biochemical assays that confirm the biotechnological potential of A. niger LBM 134 for the bioconversionof hemicellulosic substrates such as sugarcane and cassava bagasses.