Enzymatic hydrolysis of barley straw for biofuel industry using a novel strain of Trametes villosa from Paranaense rainforest

CONIGLIO, ROMINA O.; DÍAZ, GABRIELA V.; FONSECA, MARÍA I.; CASTRILLO, MARÍA L.; PICCINNI, FLORENCIA E.; VILLALBA, LAURA L.; CAMPOS, ELEONORA; ZAPATA, PEDRO D.

PREPARATIVE BIOCHEMISTRY AND BIOTECHNOLOGY

TAYLOR & FRANCIS INC

Año: 2020 p. 1 - 10

1082-6068

Agricultural practices generate lignocellulosic waste that can be bioconverted by fungi to generatevalue-added products such as biofuels. In this context, fungal enzymes are presented as an alternative for their use in the hydrolysis of cellulose to sugars that can be fermented to ethanol. Theaim of this work was to characterize LBM 033 strain and to analyze its efficiency in the hydrolysisof cellulosic substrates, including barley straw. LBM 033 strain was identified as Trametes villosa bymolecular techniques, through the use of the ITS and rbp2 markers and the construction of phylogenetic trees. The cell-free supernatant of T. villosa LBM 033 showed high titers of hydrolyticenzymatic activities, necessary for the hydrolysis of the holocellulosic substrates, hydrolyzing purecellulose to cellobiose and glucose and also degraded the polysaccharides contained in barleystraw to short soluble oligosaccharides. These results indicate that macro fungi from tropical soilenvironments, such as T. villosa LBM 033 can be a valuable resource for in-house, cost effectiveproduction of enzymes that can be applied in the hydrolysis stage, which could reduce the totalcost of bioethanol production.