Screening of new secretory cellulases from different supernatants of white rot fungi from Misiones, Argentina

CONIGLIO ROMINA O; FONSECA MI; VILLALBA L. L.,; ZAPATA PD

Mycology

Informa UK Limited, trading as Taylor & Francis Group.

Año: 2017

2150-1203

Cellulases hydrolyse the cellulose chain into single sugars efficiently. These sugars can befermented in the bioethanol process, a source of renewable energy. Misiones rainforest is oneof the most biodiverse systems on the planet subtropical ecoregions, which is the most probablesite to find new fungal strains with potential for degrading cellulose through cellulases. The aimof this work was to find an efficient cellulolytic microorganism through the exploration of nativewhite rot fungi from Misiones. From the qualitative screening 11 fungal strains were selected. Thequantitative analysis revealed that the isolated LBM 033 was the best cellulases producer,reaching 57, 226 and 387 U/l of cellobiohydrolase, β-glucosidase and endoglucanase activity,respectively. The zymograms showed that the molecular mass of most of the endoglucanasesranged from 69 to 88 kDa and the molecular mass of most of the cellobiohydrolases was 45 kDa.The search of new cellulases of secretory organisms should lead to an efficient degradation ofcellulosic materials, and thus facilitating potential applications in the production of bioenergyfrom lignocellulosic biomass.