Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase Production Using Sugarcane Bagasse as Sole Carbon Source under Light

BARCHUK L.,; DIAZ GABRIELA V; COLL PA; VELAZQUEZ E; FONSECA MI; VILLALBA LL; ZAPATA PD

International Journal of Recent Biotechnology

IJRB

Año: 2016 vol. 4 p. 25 - 34

2322-0392

Species from fungal genus Trichoderma were found to produce culture broths rich inxylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis oflignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using alow-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environmentsof Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T.atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL-1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbonsource. Besides, when experiments were carried out with light, the highest xylanase productionoccurred in shorter incubation period than experiments without it. Xylanase activity wascharacterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8.Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa insupernatants from experiment with light and only one isoenzyme of 105 KDa from experiment indarkness.