Statistical Optimization of Medium Components of Xylanase Production by Irpex lacteus BAFC 1168 strain F under liquid fermentation

DIAZ GABRIELA V; GIORGIO EM; FONSECA MI; VILLALBA L. L.,; ZAPATA PD

JOURNAL OF ADVANCES IN BIOTECHNOLOGY

Journal Manager JBT

Año: 2015 vol. 5 p. 559 - 569

2348-6201

The enhanced xylanase production of white rot fungus Irpex lacteus BAFC 1168 strain F using a liquid fermentationculture was investigated. The media nutrients were screened by a Plackett-Burman design and from the analysis ofvariance, carboxy-methylcellulose, urea and peptone were found to be the most positive and significant nutrientcomponents. Hence, these three variables were selected for further optimization using a five-level central compositedesign in response surface methodology. The validation of the model experiment was conducted reaching 8.29 U/mL ofxylanase activity when the composition medium was 1.6 g/L of CMC, 0.84 g/L of urea and 2 g/L of peptone. Optimumtemperature and pH conditions on xylanase activity were also determined using a central composite design. It wasreached a maximum xylanase activity of 8.36 U/mL when temperature and pH were 42.8°C and 5.6, respectively.