Chips and sawdust substrates application for lignocellulolytic enzymes production by solid state fermentation

ERNESTO MARTÍN GIORGIO, MARÍA ISABEL FONSECA, MARCOS RAÚL TEJERINA, ANA BELÉN RAMOS-HRYB, NOELIA SANABRIA, PEDRO DARÍO ZAPATA, LAURA LIDIA VILLALBA

International Research Journal of Biotechnology

International Research Journals Publishing House

Lugar: Sapale; Año: 2012 vol. 7 p. 120 - 127

2141-5153

White rot fungi have an enzymatic system producing o xidative and hydrolytic enzymes that act on the degradation of the cell wall components. The abunda nce of sawdust and other forestry wastes in our region, due to an important forestry activity, repr esents an interesting ecological option. These surp lus needs to be ecologically disposed. The purpose of t his study was to compare wood chips and sawdust as substrates for the production of hydrolytic (end o- β -1,4-glucanase and 1,4- β -glucosidase) and oxidative (laccases and manganese peroxidase) enzyme s secreted by five white rot fungi native from Misiones (Argentina) by solid state fermentation (S SF) We also evaluated the effect of the extraction method on enzyme recovery yield. Sawdust-SSF was pro ved to be the most suitable substrate for enzymes production. Endo- β -1,4-glucanase and laccase were the major enzymes se creted with both substrates. Peniophora sp. BAFC 633, T. villosa BAFC 2755 and P. sanguineus BAFC 2126 were the most efficient producers of both types of enzymes. G. applanatum BAFC 1168 strain F and C. versicolor f. antarcticus BAFC 266 produced mostly hydrolytic enzymes. Enzyme e xtraction was conducted in two steps to assure optimal recovery yield. A second ex traction step was necessary to sawdust SSF samples.