Influence of culture conditions on laccase production, growth, and isoenzymes patterns in native white rot fungi from the Misiones rainforest (Argentina)

FONSECA, M. I.; FARIÑA, J. I..; SANABRIA, N. I., VILLALBA, L. L., ZAPATA, P. D

BIORESOURCES

NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI

Lugar: Raleigh; Año: 2013 vol. 8 p. 2855 - 2866

1930-2126

Many biotechnological processes pursuing sustainability aim for effective, inexpensive , and environmentally friendly alternatives to replace conventional practices. Laccase - containing l ignocellulolytic systems from white rot fungi have been show n to be an effici ent enzymatic tool for ecofriendly biological treatments . One objective of t he biotechnological enzymes production proce ss is to find optimum growing and secreti on conditions for a selected fungus. In this work, different fungi isolated from the Misiones rainforest ( Coriolus versicolor f. antarcticus BAFC - 266, Ganoderma applanatum strain F, Phlebia brevispora BAFC - 633 , and Pycnoporus sanguineus BAFC - 2126) were incubated at different temperatures (25, 29, 33 °C) and pH values (3.5, 4.5, 5.5) under static co nditions for 7, 10 , and 14 days to evaluate their growing ability and laccase (Lac) production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making it necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29 ºC and pH 4.5 at the 10 th day of cultiva tion. The presence of Lac isoenzymes also depended on the pH, temperature , and time of cultivation for the different tested fungi.