Evaluación en laboratorio de un sistema sumergido de producción de endo-1,4-β-glucanasa secretada por dos cepas de hongos de pudrición blanca

ERNESTO M. GIORGIO; ANDREA MORALES; GABRIELA CONGOST, ; MARÍA I. FONSECA; CARLOS ABATE; PEDRO D. ZAPATA, LAURA L. VILLALBA

REVISTA DE CIENCIA Y TECNOLOGíA

CENTRO DE INVESTIGACIÓN Y DESARROLLO TECNOLÓGICO, FACULTAD DE CIENCIAS EXACTAS, QUÍMICAS Y NATURALES, UNIVERSIDAD NACIONAL DE MISIONES

Lugar: Posadas; Año: 2013 vol. 19 p. 18 - 23

0329-8922

Cellulose is the main component of the cell wall with great biotechnological importance regarding the analysis ofnew cellulase producers microorganisms. The objective of this study was the design and testing of a submergedproduction system of endo--1.4-glucanase using pine chips as substrate; pH and T optimum action of the enzymeextracts by two white rot fungi Irpex sp. and Phlebia sp. were checked at laboratory scale.The production system proposed in this study showed a logarithmic behaviour with respect to enzyme productionwith maximum activity of 1 U / Lh for Irpex sp.strain and 1.37 U / Lh for Phlebia sp. strain. Enzymatic activityremained constant over time through washing and adding fresh medium, simulating a fed-batch production system.Extracts of both strains showed better performance at 50°C, with higher activity at pH 3 for extracts of Irpex sp.strain and pH 4.5 for Phlebia sp. strain. This system may be optimal to start scaling up studies.