Exploring novel Penicillium lipolytic activity from the Paranaense rainforest

ORTELLADO, LAURA E.; LISOWIEC, LEANDRO A.; QUIROGA-ZINGARETTI, ADRIANA E.; VILLALBA, LAURA L.; ZAPATA, PEDRO D.; FONSECA, MARÍA I.

ENVIRONMENTAL TECHNOLOGY

TAYLOR & FRANCIS LTD

Año: 2021 p. 4372 - 4379

0959-3330

In this study, we evaluated the presence of lipases in twenty fungal strains of the genus Penicilliumusing an efficient and low-cost method with a view to an application in the treatment of cooking oilresidues. The Paranaense rainforest is one of the most biodiverse places on the planet, making it themost likely site to find new fungal strains with lipolytic potential. The objective of this study was todetermine the lipolytic potential and the isoenzyme profile of fungi belonging to the Penicilliumgenus isolated from the Paranaense rainforest. Seven fungal strains were selected usingqualitative screening. Quantitative analysis revealed that the isolate Penicillium sp. LBM 088 wasthe best producer of lipase, reaching 1224 U mL−1 of lipolytic activity. Zymogram gels of theseven selected strains showed different enzymatic profiles: In general, the molecular mass ofproteins varied from 26 to 42 kDa. Also, proteins from fungi grown on olive oil showed a highervariation in their molecular mass than proteins from fungi grown without the oil. The search fornew lipase-secreting organisms should lead to the exploitation of biodiversity in the region.