INVESTIGADORES
COCCA Claudia Marcela
artículos
Título:
Differential mechanisms of action are involved in chlorpyrifos effects in estrogen-dependent or -independent breast cancer cells exposed to low or high concentrations of the pesticide.
Autor/es:
VENTURA C; NÚÑEZ M; MIRET N; MARTINEL LAMAS D; RANDI A; VENTURINO A; RIVERA E; COCCA C
Revista:
TOXICOLOGY LETTERS
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 213 p. 184 - 193
ISSN:
0378-4274
Resumen:
It has reported that many environmental compounds may display estrogenic
actions and these findings led to researchers to associate breast
cancer risk with the use of some pesticides. The aim of this work was to
investigate the effect of chlorpyrifos (CPF) on cell proliferation and
the ERα-dependence of this action employing MCF-7 and MDA-MB-231 breast
cancer cell lines. We have also analyzed CPF action on the cell cycle
distribution and the cyclins that are implicated in G1-S and intra-S
checkpoints. Finally, the action on cell death and ROS production were
studied. We demonstrated the ability of CPF 0.05μM to induce cell
proliferation through ERα in hormone-dependent breast cancer cells. In
contrast, CPF 50μM induces intra-S arrest modifying checkpoints
proteins, through a mechanism that may involve changes in redox balance
in MCF-7. In MDA-MB-231, we have found that CPF 50μM produces an arrest
in G2/M phase which could be related to the capacity of the pesticide
for binding to tubulin sites altering microtubules polymerization.
Altogether, our results provide new evidences on the action of the
pesticide CPF as an environmental breast cancer risk factor due to the
effects that causes on the mechanisms that modulate breast cell
proliferation.