HISTOLOGICAL AND HISTOCHEMICAL STUDY OF THE DUODENUM OF FETUSES OF THE PLAINS VISCACHA (Lagostomus maximus)

TANO DE LA HOZ M.F.; FLAMINI, M. A.; DÍAZ A.O.

La Plata

Congreso; XIV CONGRESO y 11avas JORNADAS DE EDUCACIÓN; 2012

Sociedad de Ciencias Morfológicas La Plata

The objective of this work was to carry out a histological and histochemical analysis of the duodenum of mid-term and at-term gestation fetuses of Lagostomus maximus. Duodenal samples were taken at approximately 1 cm from the pylorus. Histological techniques, histochemical techniques (PAS, KOH/PA*S, PA/Bh/KOH/PAS,KOH/PA*/Bh/PAS, AB pH 2.5, 1.0 and 0.5, AT pH 5.6 and 4.2) and lectinhistochemical techniques were used. The duodenum of mid-term gestation fetuses presented short villi covered by a simple undifferentiated cubicepithelium, while the duodenal mucosa of at-term fetuses was lined by a simple cylindrical epithelium with goblet cells. Only fetuses at term presented Lieberkühn glands. The tunica submucosa of at-term fetuses had Brunnerglands. The tunica muscularis was formed by a single circular layer in the mid-gestation fetuses and two concentric layers in at-term fetuses. The duodenal histochemical profile was similar in both fetal stages. Only fetuses at midgestation showed PAS-positive granules in both enterocytes and in the traces of Lieberkühn glands. The lectinhistochemical techniques allowed identifying different residues of monosaccharide sugars evident in the glycoproteins (GPs). The histochemical techniques revealed that GPs in the duodenum of L. maximus as well as their glycosylation patterns vary according to the animals´ age.