Carbohydrate composition analysis of glycoproteins of the jejunum of the plains viscacha, Lagostomus maximus

TANO DE LA HOZ, MARÍA FLORENCIA; FLAMINI, MIRTA ALICIA; DÍAZ, ALCIRA OFELIA

Casilda

Congreso; XIV Congreso y XXXII Reunión Anual de la Sociedad de Biología de Rosario; 2012

Sociedad de Biología de Rosario

The objective of this work is focused on the analysis of the carbohydrate composition of glycoproteins (GPs) present in the jejunum of adults of L. maximus. Sections of tissue were subjected to histochemical procedures for GPs identifi cation (PAS; KOH/PA*S, PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS, AB pH 2.5, 1.0 and 0.5, AT pH 5.6 and 4.2). The AB technique at different pHs allowed the identification of two different types of goblet cells, which we called type A and type B. Type A cells were the most abundant goblet cell with carboxylated and sulphated GPs. Type B cells were found in less proportion and showed a negative reaction to AB. The GPs of both types of goblet cells presented oxidizable vicinal diol groups and sialic acid residues with and without O-acyl substitution at C7, C8 or C9 and O-acyl sugars. In comparison with the histochemical profi le of the duodenum of L. maximus, the jejunum had a greater amount of acid GPs. This study has evidenced that different GPs elaborated and secreted by the plains viscacha jejunum show a high level of histochemical complexity, related to the multiple functions carried out by the digestive tract mucus. The histochemical techniques revealed that GPs in the small intestine of L. maximus as well as their glycosylation patterns vary according to the anatomical segment of the intestine.L. maximus. Sections of tissue were subjected to histochemical procedures for GPs identifi cation (PAS; KOH/PA*S, PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS, AB pH 2.5, 1.0 and 0.5, AT pH 5.6 and 4.2). The AB technique at different pHs allowed the identification of two different types of goblet cells, which we called type A and type B. Type A cells were the most abundant goblet cell with carboxylated and sulphated GPs. Type B cells were found in less proportion and showed a negative reaction to AB. The GPs of both types of goblet cells presented oxidizable vicinal diol groups and sialic acid residues with and without O-acyl substitution at C7, C8 or C9 and O-acyl sugars. In comparison with the histochemical profi le of the duodenum of L. maximus, the jejunum had a greater amount of acid GPs. This study has evidenced that different GPs elaborated and secreted by the plains viscacha jejunum show a high level of histochemical complexity, related to the multiple functions carried out by the digestive tract mucus. The histochemical techniques revealed that GPs in the small intestine of L. maximus as well as their glycosylation patterns vary according to the anatomical segment of the intestine.