Histochemistry of the zona pellucida of the ovary of a species with natural polyovulation: Lagostomus maximus (Rodentia, Hystricomorpha, Chinchillidae)

ACUÑA, F.; TANO DE LA HOZ, M. F.; DÍAZ, A. O.; PORTIANSKY, E. L.; BARBEITO, C. G.; FLAMINI, M. A.

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2018

0936-6768

This study reports the histochemistry and the distribution of glycoconjugates (GCs) in the zona pellucida (ZP) of preantral, secondary, tertiary, polyovulatory and atretic follicles of ovaries from non‐pregnant (NPr) and pregnant (Pr) females of Lagostomus maximus. GCs were studied using histochemical and lectinhistochemical methods. The viscacha ZP was positive to all the histochemical techniques. In addition, it was observed that the intensity of staining of the ZP was constant in the different follicular stages between both female groups. The lectinhistochemical study revealed that ZP was positive for certain lectins (WGA, RCA‐I and CON‐A) and that the labeling did not vary between the different follicular stages, but between the two groups of females. By using both histochemical techniques it was established that the GCs present in the ZP label the complexity of the area. These results allow us to increase our knowledge on the biology of the viscacha´s ovary, particularly contributing to the study of polyovulation.