Protumorigenic galectins 1 and 3 are upregulated in the liver of mice exposed to continuous growth hormone levels.

DE LA FUENTE, SANTIAGO ; PIAZZA VERÓNICA G; SARRIAS, LORENA; SOTELO ANA ISABEL; BARTKE ANDRZEJ; TRONCOSO, MARÍA FERNANDA; MIQUET JOHANNA G

Buenos Aires

Congreso; XXVII Congreso ALEH 2022, Asociación Latinoamericana para el Estudio del Hígado; 2022

Asociación Latinoamericana para el Estudio del Hígado

Title: PROTUMORIGENIC GALECTINS 1 AND 3 ARE UPREGULATED IN THE LIVER OF MICE EXPOSED TO CONTINUOUS GROWTH HORMONE LEVELSAUTHORS: DE LA FUENTE SANTIAGO 1, PIAZZA VERÓNICA GABRIELA 1, CICCONI NADIA SOFÍA 1, BACIGALUPO MARÍA LORENA 1, SARRIAS LUCIANA 1, SOTELO ANA ISABEL 1, BARTKE ANDRZEJ 2, TRONCOSO MARÍA FERNANDA 1, MIQUET JOHANNA GABRIELA 11 UNIVERSIDAD DE BUENOS AIRES, CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (CONICET), INSTITUTO DE QUÍMICA Y FISICOQUÍMICA BIOLÓGICAS (IQUIFIB), DEPARTAMENTO DE QUÍMICA BIOLÓGICA, FACULTAD DE FARMACIA Y BIOQUÍMICA, BUENOS AIRES, ARGENTINA. 2 DEPARTMENT OF INTERNAL MEDICINE, GERIATRICS RESEARCH, SOUTHERN ILLINOIS UNIVERSITY SCHOOL OF MEDICINE, SPRINGFIELD, IL, UNITED STATES.ABSTRACTINTRODUCTION: Human and animal evidence revealed a link between growth hormone (GH) and cancer risk. GH excess is implicated in rodent hepatocarcinogenesis. Transgenic mice overexpressing GH (GH-Tg) develop hepatocellular tumors at old ages, with preneoplastic liver pathology similar to that observed in humans at high risk of developing hepatic cancer. Galectin 1 (GAL1) is involved in liver tumorigenesis in humans. We reported that GAL1 is upregulated in GH-Tg mice liver, even before histopathological alterations are detected, and particularly enhanced in liver tumors. OBJECTIVES: To evaluate if GH modulates the hepatic expression of GAL3, another protumorigenic galectin. As many proteins exhibit sexually dimorphic liver expression, mainly determined by distinct GH secretion patterns between males (intermittent) and females (more continuous), we assessed if GAL1 and GAL3 liver expression was affected by GH secretion pattern. METHODS: Hepatic GAL1 or GAL3 were analyzed by immunoblotting in GH-Tg mice exposed to continuously elevated GH levels, and in Swiss-Webster mice treated with GH during 5 weeks by implantation of osmotic pumps (continuous treatment) or by two daily injections (intermittent treatment). Statistics: Student´s t-test or two-way ANOVA; P