Decarboxylase activity involved in methyl ketones production in Staphylococcus carnosus 833, a strain used in sausage fermentation

SILVINA GRACIELA FADDA; A LEBERT; S. LEROY SÈTRIN; R TALON

FEMS MICROBIOLOGY LETTERS

FEMS

Lugar: Reading (UK); Año: 2002 vol. 210 p. 209 - 214

0378-1097

Staphylococcus carnosus strain 833, inoculated into sausage, increased the levels of methyl ketones which contributed to the cured aroma. These ketones were predicted to arise from incomplete L-oxidation followed by a decarboxylation. To check this hypothesis, we measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a aroma. These ketones were predicted to arise from incomplete L-oxidation followed by a decarboxylation. To check this hypothesis, we measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a strain 833, inoculated into sausage, increased the levels of methyl ketones which contributed to the cured aroma. These ketones were predicted to arise from incomplete L-oxidation followed by a decarboxylation. To check this hypothesis, we measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a L-oxidation followed by a decarboxylation. To check this hypothesis, we measured the L-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate aL-decarboxylase activity in resting cells of S. carnosus grown in complex or in synthetic medium, using as substrate a L-ketoacid, which can be an intermediate of the L-oxidation pathway. This activity was present throughout the growth period. The enzyme appeared to be constitutive because no induction was observed. High aeration, a pH of 5 and the presence of nitrate promoted the production of methyl ketones. / 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. enzyme appeared to be constitutive because no induction was observed. High aeration, a pH of 5 and the presence of nitrate promoted the production of methyl ketones. / 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. -ketoacid, which can be an intermediate of the L-oxidation pathway. This activity was present throughout the growth period. The enzyme appeared to be constitutive because no induction was observed. High aeration, a pH of 5 and the presence of nitrate promoted the production of methyl ketones. / 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.