Genome- Wide characterization of Dof gene family in peanut (Arachis hypogaea)

SAMOLUK SS; JOSÉ G. SEIJO

Congreso; XII Argentinean Congress of Bioinformatics and Computational Biology; 2022

ABSTRACTBackgroundThe Dof gene family encodes a group of plant-specific transcription factors. Studies of these genes in someagronomic important crops have revealed that play a key role in plant growth, development, and responseto biotic/ abiotic stresses. The recent publication of peanut genome and their wild diploid progenitorsprovides a great opportunity to perform genomic approaches for a better understanding of the regulationof many important traits, a crucial step for crop improvement. In the present study, we report a genome-wide analysis of Dof genes in A. hypogaea (AhyDof), including phylogenetic inferences, gene structures,chromosomal locations, gene duplications events, and the analysis of expression patterns.ResultsA total of 61 full- length genes (AhyDofs) across 20 chromosomal pseudomolecules of peanut genomewere detected. Although the deduced AhyDof proteins were variable in length, molecular weight andisoelectric point, they showed a highly conserved zinc finger structure, characteristic of this family.Phylogenetic analysis revealed the existence of different groups of sequences with particular distributionpatterns of protein domains, as well as exonic and intronic structures. Genome- scale analysis of syntenyindicated that segmental duplication events may have played an important role in the evolution of theAhyDof genes. Analysis of cis- acting elements in the promoter region revealed the presence of motifsassociated with response to hormones, light and stress conditions. The expression levels of these geneswere variable in the different tissues and developmental stages of the plant.ConclusionsThis study provides insights into the evolution and functional diversification of AhyDofs as a basis forfuture research in cultivated peanut.