Antitumoral effects of Pleurotus ostreatus i-fraction in breast cancer

RAMBURGER ; FERRONATO ; GUEVARA; CUESTAS JUAN MANUEL; POSTEMSKY PABLO; CURINO ; FACCHINETTI ; ALONSO ELIANA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC),; 2021

LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC),

1) Mushrooms are considered as "small pharmaceutical factories" producinghundreds of bioactive compounds, many of which have shown to exertantitumoral activity in different types of cancer. Argentina has a high mushroomsdiversity and important scientific-technological development applied to itscultivation. However, the antitumoral phytotherapeutic potential of ediblemushrooms cultivated in our country has not yet been considered. In this context,the purpose of the current study is to determine the antitumoral activity in breastcancer of Pleurotus ostreatus I-Fraction, an extract of water-solublepolysaccharides obtained from fruiting body, initially evaluating its potentialimmuno-independent antitumoral activity. To achieve the proposed objective, weemployed a murine mammary adenocarcinoma 4T1 cells line and performed cellviability assays by colorimetric assay with crystal violet, cell cycle analysis by flowcytometry, and wound healing assay. We found that P. Ostreatus I-Fraction atconcentration from 2.5 mg/mL and ranging from 1,0 to 2,5 mg/mL decreased theviability of 4T1 cells in a concentration-dependent manner, at 24 hours and 48hours respectively (p