In Vitro Studies of Secondary Metabolite?Related Responses in Some Species of GenusGrifola (Agaricomycetes) from Argentina

POSTEMSKY P.D.; CURVETTO N.R.

INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS

BEGELL HOUSE INC

Año: 2016

1521-9437

ABSTRACT:Grifola gargal Singer andGrifola sordulenta (Mont.) Singer mushrooms are related to Grifola fron-dosa (Dicks.) Gray, which is well known for its medicinal properties. In vitrostudies were performed to find a useful guide for optimizing the environmental parameters through biotransformation of lignocellulosic materials and basidiome development, also considering secondary metabolism?related responses (SMRRs) associated with these processes and the variability among species and strains; this optimization is necessary to make the mushroom?s indus-trial cultivation profitable. Morphological features of mycelial cultures revealed that intraspecific variability was of taxonomic relevance. A low ligninolytic capacity in studied Grifola species was observed when compared with 2 control species: G. frondosaand Ganoderma lucidum. Experiments with nutrient media containing different carbohy-drate sources indicated that G. gargalmycelia grew better in xylulose and G. sordulenta, in xylulose or cellulose; in addition, the latter species presented cellobiose dehydrogenase activity. An additional study of SMRRs under different light conditions (aroma, pigmentation, and morphogenic manifestations) showed that white light was better than blue, green, or red-filtered light at inducing advanced SMRRs. The results of SMRR stimulation are proposed as useful guidance for optimizing the environmental parameters for bioprocesses aimed at metabolite production